Leucyl-tRNA synthetase

Protein-coding gene in the species Homo sapiens
LARS1
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

2WFD

Identifiers
AliasesLARS1, Leucyl-tRNA synthetase, ILFS1, HSPC192, LARS, leucyl-tRNA synthetase 1, LRS, LEUS, LFIS, PIG44, hr025Cl, LEURS, RNTLS
External IDsOMIM: 151350; MGI: 1913808; HomoloGene: 7083; GeneCards: LARS1; OMA:LARS1 - orthologs
Gene location (Human)
Chromosome 5 (human)
Chr.Chromosome 5 (human)[1]
Chromosome 5 (human)
Genomic location for LARS1
Genomic location for LARS1
Band5q32Start146,110,566 bp[1]
End146,182,696 bp[1]
Gene location (Mouse)
Chromosome 18 (mouse)
Chr.Chromosome 18 (mouse)[2]
Chromosome 18 (mouse)
Genomic location for LARS1
Genomic location for LARS1
Band18|18 B3Start42,335,363 bp[2]
End42,395,259 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • Brodmann area 23

  • endothelial cell

  • ventricular zone

  • middle temporal gyrus

  • body of pancreas

  • right uterine tube

  • ganglionic eminence

  • primary visual cortex

  • cartilage tissue

  • islet of Langerhans
Top expressed in
  • medullary collecting duct

  • basilar part of occipital bone

  • lacrimal gland

  • yolk sac

  • primitive streak

  • renal corpuscle

  • calvaria

  • seminal vesicula

  • ureter

  • salivary gland
More reference expression data
BioGPS
More reference expression data
Gene ontology
Molecular function
  • aminoacyl-tRNA ligase activity
  • nucleotide binding
  • leucine-tRNA ligase activity
  • ligase activity
  • protein binding
  • ATP binding
  • aminoacyl-tRNA editing activity
  • valine-tRNA ligase activity
  • GTPase activator activity
  • glutamine-tRNA ligase activity
Cellular component
  • cytosol
  • nuclear body
  • cytoplasm
  • lysosome
  • endoplasmic reticulum
  • endomembrane system
  • aminoacyl-tRNA synthetase multienzyme complex
Biological process
  • tRNA aminoacylation for protein translation
  • protein biosynthesis
  • leucyl-tRNA aminoacylation
  • valyl-tRNA aminoacylation
  • protein targeting to lysosome
  • regulation of cell size
  • negative regulation of autophagy
  • cellular response to amino acid starvation
  • positive regulation of GTPase activity
  • cellular response to amino acid stimulus
  • cellular response to leucine
  • positive regulation of TORC1 signaling
  • cellular response to leucine starvation
  • glutaminyl-tRNA aminoacylation
  • aminoacyl-tRNA metabolism involved in translational fidelity
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

51520

107045

Ensembl

ENSG00000133706

ENSMUSG00000024493

UniProt

Q9P2J5

Q8BMJ2

RefSeq (mRNA)

NM_020117
NM_001317964
NM_001317965
NM_016460

NM_134137

RefSeq (protein)

NP_001304893
NP_001304894
NP_057544
NP_064502

NP_598898

Location (UCSC)Chr 5: 146.11 – 146.18 MbChr 18: 42.34 – 42.4 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Leucyl-tRNA synthetase, cytoplasmic is an enzyme that in humans is encoded by the LARS gene.[5][6]

Function

This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginyl-tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known.[6]

Interactions

Leucyl-tRNA synthetase has been shown to interact with EEF1G.[7]

Inhibitors

See also

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000133706 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000024493 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Giles RE, Shimizu N, Ruddle FH (September 1980). "Assignment of a human genetic locus to chromosome 5 which corrects the heat sensitive lesion associated with reduced leucyl-tRNA synthetase activity in ts025Cl Chinese hamster cells". Somatic Cell Genetics. 6 (5): 667–687. doi:10.1007/BF01538645. PMID 6933703. S2CID 11128003.
  6. ^ a b "Entrez Gene: LARS leucyl-tRNA synthetase".
  7. ^ Sang Lee J, Gyu Park S, Park H, Seol W, Lee S, Kim S (February 2002). "Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex". Biochemical and Biophysical Research Communications. 291 (1): 158–164. doi:10.1006/bbrc.2002.6398. PMID 11829477.
  8. ^ Markinson B, Ghannoum M, Winter T, Rycerz A, Rock F, Gupta AK (January 2018). "Examining the Benefits of the Boron-Based Mechanism of Action and Physicochemical Properties of Tavaborole in the Treatment of Onychomycosis". Journal of the American Podiatric Medical Association. 108 (1): 12–19. doi:10.7547/16-154. PMID 29547036.

Further reading

  • Norcum MT (August 1991). "Structural analysis of the high molecular mass aminoacyl-tRNA synthetase complex. Effects of neutral salts and detergents". The Journal of Biological Chemistry. 266 (23): 15398–15405. doi:10.1016/S0021-9258(18)98629-1. PMID 1651330.
  • Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–174. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
  • Quevillon S, Robinson JC, Berthonneau E, Siatecka M, Mirande M (January 1999). "Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein". Journal of Molecular Biology. 285 (1): 183–195. doi:10.1006/jmbi.1998.2316. PMID 9878398.
  • Rho SB, Kim MJ, Lee JS, Seol W, Motegi H, Kim S, Shiba K (April 1999). "Genetic dissection of protein-protein interactions in multi-tRNA synthetase complex". Proceedings of the National Academy of Sciences of the United States of America. 96 (8): 4488–4493. Bibcode:1999PNAS...96.4488R. doi:10.1073/pnas.96.8.4488. PMC 16359. PMID 10200289.
  • Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O (February 2000). "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 7 (1): 65–73. doi:10.1093/dnares/7.1.65. PMID 10718198.
  • Kang J, Kim T, Ko YG, Rho SB, Park SG, Kim MJ, et al. (October 2000). "Heat shock protein 90 mediates protein-protein interactions between human aminoacyl-tRNA synthetases". The Journal of Biological Chemistry. 275 (41): 31682–31688. doi:10.1074/jbc.M909965199. PMID 10913161.
  • Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, et al. (October 2000). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Research. 10 (10): 1546–1560. doi:10.1101/gr.140200. PMC 310934. PMID 11042152.
  • Sang Lee J, Gyu Park S, Park H, Seol W, Lee S, Kim S (February 2002). "Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex". Biochemical and Biophysical Research Communications. 291 (1): 158–164. doi:10.1006/bbrc.2002.6398. PMID 11829477.
  • Ling C, Yao YN, Zheng YG, Wei H, Wang L, Wu XF, Wang ED (October 2005). "The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex". The Journal of Biological Chemistry. 280 (41): 34755–34763. doi:10.1074/jbc.M413511200. PMID 16055448.
  • Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, et al. (January 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
  • Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
  • Maeso E, Rueda A, Jiménez S, Del Hoyo P, Martín R, Cabello A, et al. (May 2007). "A novel mutation in the mitochondrial DNA tRNA Leu (UUR) gene associated with late-onset ocular myopathy". Neuromuscular Disorders. 17 (5): 415–418. doi:10.1016/j.nmd.2007.01.015. PMID 17363246. S2CID 26750219.
  • Lue SW, Kelley SO (April 2007). "A single residue in leucyl-tRNA synthetase affecting amino acid specificity and tRNA aminoacylation". Biochemistry. 46 (15): 4466–4472. doi:10.1021/bi0618215. PMC 2518062. PMID 17378584.
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